Prep HPLC Column, HPS Silica, 5um, 120A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Info:
75001-P250 Cogent HPS Silica™ Prep HPLC Column is packed with high‑purity, unmodified silica, delivering consistent and reproducible performance for large‑scale purification of polar and hydrophilic compounds.
Because the stationary phase is unbonded and unmodified, it provides strong silanol interactions, making it highly effective for:
Normal Phase chromatography (primary mode)
HILIC separations for polar analytes (highly suitable secondary mode)
This dual‑mode capability allows maximum flexibility in method development, enabling both non‑polar solvent systems (NP) and high‑organic HILIC conditions for optimized separations.
This proprietary HPS Silica™ has been a very popular, premium‑value phase used by HPLC laboratories for many years, offering a dependable alternative to columns such as Inertsil® and Luna® with consistent selectivity and reproducibility.
The 5 µm particle size is ideal for preparative workflows, providing:
Lower backpressure vs smaller particles
Higher allowable flow rates
Robust performance for repeated purification runs
250 mm Length – Maximum Resolution Preparative Separation
The 250 mm length provides the highest resolving power in this preparative format.
Maximum resolution compared to 50 mm, 100 mm, and 150 mm columns
Superior separation of closely related or co‑eluting compounds
Highest peak capacity for complex mixtures
Extended retention for improved purity
✅ Recommended for final purification, impurity isolation, and the most complex separations
21.2 mm ID – Preparative Scale
Designed for preparative HPLC purification
High sample loading capacity
Suitable for analytical‑to‑prep scale‑up
Compatible with large‑volume injections and fraction collection systems
✅ Recommended for process development and production‑scale purification
Flow Rate & System Requirements (21.2 mm ID, 5 µm)
⚠️ Requires preparative or process‑scale HPLC system capability
Typical flow rate range: ~15 – 40 mL/min
Moderate-to-higher backpressure due to 250 mm length
Designed for high‑flow purification
Compatible with preparative detectors and fraction collectors
✅ Important: A specialized preparative HPLC system is required for columns of this size
Why Choose HPS Silica™ for Preparative HPLC
Unmodified Silica – Dual Mode Capability
Ideal for Normal Phase separations
Highly suitable for HILIC separations of polar compounds
High‑Purity Silica Surface
Strong retention for hydrophilic analytes
Consistent, reproducible selectivity
USP L3 Compatibility
Suitable for compendial and validated methods
Reliable alternative to legacy silica columns
Proven Performance
Widely used across preparative laboratories
Long‑term, dependable results
Performance Advantages
Low Backpressure (5 µm Particles)
Enables high flow rates for preparative purification
Compatible with standard prep systems
Fast Equilibration
Improves throughput in large‑scale workflows
Flexible Selectivity
Supports both Normal Phase and HILIC separations
Typical Applications
Large‑scale preparative purification of lipids and fats
High‑resolution normal phase separations
HILIC purification of polar compounds
Impurity isolation and final purification
Pharmaceutical and chemical production workflows
Important Usage Notes
Suitable for Normal Phase and HILIC chromatography
Use either non‑polar solvents (NP) or high‑organic mobile phases (HILIC)
Requires preparative or process‑scale HPLC system
Increased system pressure expected due to longer column length
Ensure proper equilibration for reproducibility
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable